TARGET CSIR NET JULY 2025 with Me I Biochemistry I Lecture 01 I DEC 2024 PYQ Solutions IIFAS

0:11 good morning friends I hope my voice reaches you It must be coming clearly. Good morning Akansh. Akanshika good morning please have it akkansha pal alfizza deepshika monica beniwal Good good morning children. Bhavna ji good Morning and I request all of you who have come Some kids don't know the timing If yes then please share the link with them So that he can also connect. specially yours Friends who are preparing for CSR Are. Yes Sakshi this is the first class. Ankita good morning Manisha good morning Priya Sambhavi Pawan Mahak Life is Science. Good Morning. Megha Sharma ji good morning. yes good morning parvinder shaikh

1:03 No problem Sunita, this time we are 99% sure I will bring it. good morning heena ji good Morning Jyoti Ji Good Morning Meghna Tanvi Reena Christmasman Vine Good Morning Rajshri Rajnashree Good Morning Vaishali Kiran Jamuna Sunita Science Doll Kamlesh Savitri Rani Kumari Peace is good for everyone morning sleep because you guys are here But many people get very short notice because people wouldn't even know that today's what time is the session so please please tell them Please inform me because before 11:00 It was scheduled at 8 o’clock. schedule it now at 9:00 has been done because my further There were overlapping classes. that's why Good Morning. Good morning Kiran. Good Morning Soumya. Good morning Priyanka.

1:49 Good morning Raju. Good Morning. Good Morning everyone. Very very good morning. If only 8 units are completed then it is very ok right? What more do you want, son? Eight units have been completed. what else do you want To you? Very good 8 units completed So good morning Meghna ji Neha ji 8 units are very sufficient, just the rest Connect with us now for your exam Mapping and this series which starts today It has started, use it only Definitely you will benefit, thank you Hello Deepshika Kavita ji Aarti ji Raju good morning kids good morning Good Morning Mansi

2:38 Good morning Meghna, it is getting late in the evening isn't it 5:00 class meghna double biology chal There are two overlapping classes together I can't drive, that's why I did it in the morning Isn't it, son? 12 minutes of rain that's good in the evening The class is at 5:00, right son? That's why I didn't come at 5:00, Vishal Sir by I have clearly said sir my class is disturbed there shouldn't be Good Mansi good good morning. So hello friends and good morning to each one And everyone. very very very good morning and Welcome to the session. I am hoping my The sound must be reaching you clearly. I am sorry for coming with a very short notice because Most people are concerned about timing There might have been some confusion. but due to me

3:26 The classes were back to back. which schedule I have brought it for you from what is available Am. And before I move on, let me tell you about yesterday The class will be at 8:00am. 1 more hour First. Ok? Today I am in such a hurry I didn't want to because then you guys would know that I can't do it. yes this will be considered complete probably sheikh and We have nothing to do with that. YouTube's involvement. If you are a YouTuber If you become a Gold Member then from the day you Your 1 month starts from there It is counted. Ok? and automatically if you won't take it for next month The subscription will be cut, that's not it Issue. yes absolutely you are teasing vishal sir Can't do it. Vishal sir's class in the evening It will remain as it is at 5:00. Because double

4:13 Biology has also emerged out of a very interesting race. It has been happening. Coming out of a very interesting phase It has been happening. So we neither know Drosophila nor I I am also afraid of Drosophila. Also from Frog I am afraid. I am also afraid of Seelighan. and red We will have to be afraid of it. Where is the escape from Mammal? Can I go to tax? So Vishal Sir's class It hasn't been disturbed at all as of now. Correct Is. Let us go Yes, classes will start from tomorrow at 8:00 am. Ok. Ok. Ok. Its ok Deepshika ji. So the session Let's start. I hope that all of you may have shared this link. Some? Reasons Straight with Your Friends It must be forwarded. can happen on short notice It is possible that he could not come. So I want to so that the information reaches them also that yes brother The session has started and it is very It is an important session. so hello friends

4:59 Basically, in this session of ours, we in Biochemistry December 2024 Around PYQ Questions We will study the concepts. The purpose is very simple The thing is. Like last time also we did P.Y.Q. I did it. So he asked her a lot of questions Concept questions in next exam He appeared in. This time too, this is mine I believe we have some at least last six Check out the PYQ of the month and get a If you look six months old, it will be very good Will be done. what will happen to that that questions Generally people will come around to it. About three to four questions from each unit in Part B Questions come and in part C Five to six questions come. If we have these If PY PYQ is also targeted then I am sure that you will understand these concepts.

5:46 On this basis, two questions of Part C are made Are. If two questions are made from each unit So what else do you want brother? and then part B It will become two questions or one question. He And there are more benefits. Ok? Come on let's start We do it. Let us go. So friends, before I proceed further I would like to tell you Let me tell you that the crash course has started And in the same session two units are also completed It is done and from today I am studying Genetics I am starting it at 11:00 that's mine The session is about to happen. that's why i am Little bit late. But you got two units It will be found in the recording. you want So we can fix that too. If you Rapid revision of things in short time if you want to do it and if you are with us only I am studying from YouTube and only online By giving mock test or regular unit wise Check your practice by taking tests If you want to do it then yes, do CSR Net

6:32 The test series has also been launched recently. Take advantage of it for two months with 70% off You can pick it up. friends this book is I hope you already have it It will be. But if it is not there then let me tell you that brother You must have this book because In this December 2024, we will answer the questions We are discussing this, it should also be included in this Have gone. You can also buy this from Amazon or Flipkart Or you can purchase from our website. And the pointer book of exam mapping is now available for print You can pre book it available with us also I had done. Available with us in print Has been. And now this book has also started being dispatched It's done. So hopefully within 8 days Five to 7 to 8 days depending upon your Locations This book will start reaching you will go. Ok? and i can believe and i

7:22 can also assure you that this book is as much as you This book is giving you the same benefit You are also going to give this upcoming exam In. So good morning everyone. Good Morning Ankita, Somu, Ritu, Rekha, Deepshika, Pooja, Dinesh, Rani, Hemas Good Morning. Good morning children. Good Morning. Okay friends? and who, my learned friend, We are not targeting this year's exam are targeting the next exam I am waiting for the December one. So for them today The batch has started from now itself. today on 16 june It has just started. You can also take advantage of this and the name of the batch itself is Great Taste of It is all the time. because this is the best time

8:09 To Register If You Are Targeting the December Schedule. Because I will get it back in 5 years Will go. Otherwise the train will be late. But those who are preparing for this time I am with you on YouTube. So good morning Pooja. Good Morning Neha Rani Rahul Jyoti Laxmi Abhishek Ajay Everyone agreed, even the goat is here, so friends Welcome to IFS platform and I am I have told you that today we are going to discuss Biochemistry is important Studying concepts and important concepts After that we start trying to solve the question We will try and see if they can do that to us The question is whether it is being made or not I am getting it. I'm quite sure that you Solve the question and you will make sure about the concept After reading. Friends, first of all we The question which is the simplest concept

8:56 I am reading That is a concept on Molecular Interactions. In this way two molecules are combined together Let's interact. like proteins dna How do DNA, DNAse and lipids interact? Are you interacting? with lipid member. How are the proteins interacting? His We are talking about it. Friends, Molecular interactions are divided into two broad Can be divided into categories. this could be Covalent bonds and these could be variety of non-covalent bonds. Friends, where Anywhere you will see that the primary The structure will be, that primary structure Wood can be stabilized by covalent bonds. Covalent bonds tightly bind two molecules They hold it together. Covent Bond Is formed by sharing of electrons. Covalent bonds are comparatively very

9:41 Stronger as compared to rest of non Covalent Interactions. about 10 times or They are even stronger than that. There are covalent bonds. they form a primary Structure of Bio Molecules. For example peptides are usually formed between amino acids bond or glycosidic bond in sugar Talk about bonds or inside lipids Let's talk about Easter Bond. all of them R. Covent Bond. Although covalent bonds Dice remain even after protein folding Bonds etc. can be seen. butt give R comparatively rare. Mainly they stabilize the primary structure Friends, electrostatic interaction remains which interactions are electrostatic Hydrogen bond hydrophobic dies Interactions Are Referred to as Non-Covalent Interactions are called non-covalent interactions which in biological systems

10:26 It is comparatively weak. non covalent Electrostatic interactions Changing the interaction name is a plus There is a charge and there is a minus charge between them The attraction that I have is in those attractive ways What will happen? Yes, if the two molecules are in the same way There is a charge of . This is also protein positive It is charged. These proteins are also positively charged Is. Then there could also be repulsion force. One stand of DNA is negatively charged. The second stand is also negatively charged by DNA Is. So there should be repulsive force between them as well. Can. But we basically call molecules Making them interact with each other. For them If you want to connect then between them What should the F be like? being attractive Needed In electrostatic attractive Fe Charging is a must. a positive charge, Negative charge on the other. many such Examples are what we call electrostatic Interactions can be considered. such as dna

11:11 and stone proteins. Phosphate in DNA There is negative charge because of the group. And lysine at various places in the stone protein and arginine are found. and lysine and The amino group in the side chain of arginine is which bears a positive charge on the PA. So with negatively charged DNA Positively Charged Histone Proteins Intact He does. Ok? listen carefully Friends. so in the same way enzymes and In many places in the substrate also you will find Electrostatic interactions can be observed Can be done. Isn't it? a You have an active site There is a positively charged group in it. Let's From aspartic acid or glutamic acid. So You have no positive charge near the substrate There could be a charge group that comes with it He must be interacting. friends other than also electrostatic interaction and An example can be seen in the peripheral

11:58 with membrane proteins and lipids that Removed by giving a simple treatment of salt May go. So remember where Can be seen in the compliance system enzymes in dna stone proteins peripheral membrane proteins in the substrate and between lipids. these are the examples of electrostatic Interactions. Ok Supriya? Even if this video is hidden So keep it in your mind so deeply That It doesn't fly out of your mind. Which one of us is ours This video needs to be saved for kids. We are only talking about this exam, right? It will be available to you till then. don't worry hai No. Then we're talking about hydrogen. About bonds. Hydrogen Bond Edge its name suggests it is formed between two Electronegative atom is one of the atoms Act as a hydrogen donor and another act

12:44 As a hydrogen acceptor. We also say that can hydrogen bond is between a Hydrogen atom and other electronegative Atom. There are many examples of hydrogen Bond's too. Such as Liquid state of water or state of water ice Is. What is the reason? Hydrogen bond. Is No? You remember this. There is no one in this There shouldn't be any problem. Maharashtra Set The analysis will be out by 3:00 pm today. I hope so because everything is very My son is busy, I can understand the condition of teachers which come in offline batches. That same The method that best explains the hydrogen bond Example is ATK in double stranded DNA forms a double hydrogen bond with it. G C It forms a triple hydrogen bond with . which are the secondary structures of RNA

13:29 whether it is a hair pinal structure or a color leaf structure that is also stabilized By hydrogen bonding. Even Joe biological members are their polar heads and Polar head in the middle of the water and many more electrostatic interaction between proteins Hydrogen bonding is also seen Can be got. Hydrogen Bonding Even Stone can also be seen in proteins and DNA Can be got. Specially Minor Group of DNA and stone can also be present in proteins Is. But major interaction between DNA and stone inside we will say that is an electrostatic Interaction. Yes Singh sir you will definitely get it. Friends, let's talk about hydrophobic interactions. I am talking. Hydrophobic interactions between those molecules which are non-polar

14:16 and non polar molecules when dissolved in water is suspended then you see that those molecules aggregate together Are. The purpose is to exclude water Could. and thermodynamical concept According to this calculation also we can say that when the non Polar molecules aggregate with each other then the entropy of water increases Which makes the process favourable. There are also many benefits of hydrophobic interactions This is an example. Like lipid by layer. The nonpolar tails in the lipid bilayer are interconnected I move away from the water and It interacts with me. that is due to Hydrophobic interactions. we say that self The sealing nature of a membrane is due to Hydrophobic interactions. so in the membrane Look, there is also electrostatic interaction. Hydrogen bonding also occurs inside the membrane Is. Both of them have hydrophilic interaction

15:02 It is said. inside the tail of the membrane There are also hydrophobic interactions. His Besides friends, hydrophobic interaction is Responsible for protein folding. Secondary Structure to Tertiary Structure which is protein that is basically due to A Hydro Hydrophobic Interactions. Friends, apart from him the weakest There is interaction that is wonderful interactions. One Role Interactions It is the Weakest interaction it can occur between any two Nonbonded atoms Any two nonbonded atoms When atoms come in contact with each other induce dipoles on top of each other and the induced dipole between them Induced dipole interaction occurs. just this This is called the Wonder Wall interaction. Friends when they are in contact with each other then

15:49 distance is that is called as wonder wall Contact distance: If you take him away The attractive effect will decrease. if you bring it close Attractiveness will increase. but non bonded Atom who knows his wonder contact distance and Bring it with you. Brought them closer. Wonder From wall contact distance to near Come. then the overlapping of electron shells and the overlapping of electron shells will be instead of being attractive because of being here But the repulsive force will operate. What will happen? Repulsive force will operate. Ok? Come on friends if you understand this So now we are ready to ask some questions For those who appeared in the previous exam. This Answer the question. Very good bma. You have separated from me.

16:34 So this is a very good thing. So now these Answer the questions in this happiness. thank you You aspirants sir. I too love you all. And Many children do not know time Because of this he called this session You might not have joined. So please you do this Share the video link so that he can also be able to connect and benefit them too Because everyone's target is the same, Amar. everyone's Target JRF and seats are around 1500. We all prepare together. Let us go Exactly what I am saying is a theory I have to learn. because only we Do not look at PYQ questions. We have to remember the theory surrounding it. Now look at the questions one by one, I will also I will solve this too. you solved it Took it. I will solve it too. who

17:19 Congratulations to all those who gave the correct answer. V Type of interaction primary continuum Stability of nucleosomes. Isn't it? See The word is written primary. This is very It is important that you do not lodge any objection Could I get it done? because secondarily something else also It is possible that the tertiary level could be something else Can. you can say sir wonder wall There could also be interaction. tertiary then Anything can happen. primary wrote one word Has gone. So hydrogen bond between DNA base pair and serine thiony residue of This is not it is not primary. it is us You can say it could be secondary Reese. It cannot be primary reason. Water Wall interaction or we can say tertiary Interaction may occur. number three position But it can be kept. primary first It cannot be kept at this place. hydrogen bond between dna phosphate back

18:05 Bone and Main Chain of Stones. no so It doesn't happen at all. Electrostatic Interactions Involving the DNA Phosphate Backbone and lysine residue of stone. yes this It is most important because it is inside the DNA It is negative and occurs in children Positive charge. Did you understand it? See Next time this question will come which ones Is the force responsible? so the force is Responsible children hydrogen bonding too Is. Even wonder interactions play a role Are. Even hydrophobic interactions play a role and electrostatic Interactions also play a role. But It is said here that of these Which interaction in nucleosome? So There are two interactions in the nucleosome. One is mainly electrostatic and The second is hydrogen bond. in which Electrostatic plays a dominant role Is. It is a secondary role, what could that be?

18:52 Hydrogen bonding can occur. so to all Greetings. Good morning everyone. all to all Welcome to today's session. friends on this concept There is another question. you will see the kids last Inside the Bar Exam. on the concept The question was asked in the morning shift Almost the same concept in evening shift too Was asked. So an attempt was made to Balance should be maintained. Although, it is a different matter that The level of the evening paper had increased a bit. But you will find that one question is the same Placed in the morning sheet on concept. One Evening shift on question same concept An attempt was made to keep it in also. g book to Priya, it has just been dispatched. where from now Will I get it son? It will take time. it will take Reach you in five to six days. Right now you have Had pre booked. We have it in print tomorrow

19:37 She has come. She came the day before yesterday. We have dispatched since yesterday have started doing it. it will reach to you in Its short duration. Chalo very good Kartikeya. Everybody needs to answer. Why is everyone else watching, brother? Answer Why are you not giving it? Only this PYQ session is the last we will first Studying the concepts. and then we are Going for PYQ. Directly Not going to PYQ. you give the answer have been. I just read you the theory first I have been. Which of the following property is not. Keep in mind that it says not Responsible for self sealing nature. Self What is not responsible for sealing nature Is? absolutely an empathic character Will be an empathic character. only then non It is the polar tail that will come inwards. yes It is an empathic character. for the same reason There will be hydrophobic interactions. A

20:24 Hydrophobic interaction between Yes Tails came in from empathic nature and Which interaction is developed here? Has it started happening? Children develop inside them It started happening. which one here Interaction starting to develop? Hydrophobic interactions. exactly this thing That is also correct. Hydrogen bonding between Yes This can also happen. Absolutely. which here Interactions will occur with water molecules That wood be hydrogen bonding. This also be Can. It can definitely happen. which This can be called a hydrophilic interaction. It is absolutely possible. This is also possible. Water With polar head. Hydrogen Bonding Still working. The tails are hydrophobic among themselves are interacting. Tail inside Why are you coming? because the lipids are empathic Oh Deepshika beta, be careful this time covalent interaction between a lipid molecule oh brother no no no covent

21:11 If interactions occur within lipid molecules then neither the lipid would be mobile nor There would be no such thing as fluidity Ok yes this is the same class son which is strategy Wali I told you ok baby Children look at the next question, look at another one, how many The first topic of biochemistry is awesome The question is coming in the morning as well as in the evening question is coming and third question is coming is happening due to molecular interaction which one of the following bonds supports the Three Intertwined polypeptide strand in triple helical Structure of collagen, there are three types of collagen Strands are a strand of collagen

21:56 this is the second strand of collagen and this collagen which is the third strand in between Does interaction role play? so will you tell me Which interaction is there between them interactions will occur that will produce different collagen Which is obviously inside the strands If covalent bonds exist then who cares? will play an important role Where can I get the PDF where Vishal Sir gives it There is a PDF, you will get its PDF there as well Son ok well done yes very good so what is the answer for this Hydrogen bonding is done, well done kids! So hydrogen bonding is a non-coal interactions that are secondary to the protein is also seen in the structure and This was also seen in the triple helix of collagen

22:42 May go. Well done Friends, now we will read the question of PA about. Do you know the formula of PA? PA = - log h+ - log h OK? this is it Everyone knows. That means you will get concentration of hn If given in molar format then in full Place it. You can get the log of that from your PA will come nearby. Everyone knows. Friends when Sometimes there are two solutions, suppose one of pH2 is a solution and any other solution of PA3 is They have to be mixed together. Resultant What will be the PA? So take care of it directly Don't take the average. What do you have to do? The solution one has H2O as compared to its PA. The ion has to be removed. which is solution to How many H ions are there in it from its PA Have to take it out. By adding the H ions of both

23:30 Average can be calculated. So you have an average hn will come and pay back from that average hn Can be removed. In logarithmic scale If there are values ​​then add them directly Average cannot be calculated. So first Convert them to normal scale. Now you tell me sir how will i calculate hn? So hn if the formula for pH is - log hn then hn The formula is hn = anti-alpha which gives the pa Is put a minus sign in front of it and take its anti what will come to you h ion The concentration of solution one is also The solution concentration will come in molars to hn concentration will also come in molar Add the hn concentration of both the solutions do it

24:15 And the total volume is obviously both If taken in equal amount then its average can be extracted and returned from this average H PA can be removed. This thing in three steps It will be. yes Mahakash sir hello rupee rule now friends one more if you have a molecule and that brings about a change in pH e.g. An acidic molecule is acidic if it is in PA If he brings change then how much will he bring in PA He is bringing changes on the basis of his Concentration can also be found out. Now suppose we have a molecule. Suppose any one mole, say salicylic acid

25:02 Is. One we have a molecule. For him As soon as you add it to a solution So he changes the PA from 7 to 6 Inside. If it becomes a complete dissociation Is. This means that we get to know this indirectly How many H ions did it donate? HN know how Will you apply it? So how many HN were there in the solution We came to know about that. Solution Six I found out how many HN I have. HN how Will you take it out? HN = anti-PH. So Solution How many HN were there in the beginning? PH6 happened So how many HNs were there? Obviously if PH is 6 then HN The consideration increased. so first of all first of all You have to find out how much amount of HN increased. As much the amount of H2O increases, so much

25:50 amount of salicylic acid because we assume if that's salicylic acid 100 If there were molecules then there were 100 molecules When salicylic acid is broken down, 100 H ions are obtained Will be. The number of H ions added is equal to the number of PA There might have been a change in it. very simply From the concept we can understand the properties of that molecule. You can also find out the concentration indirectly Are. So for example PA became six. When to Drink If consumption was there then what was the concentration of HN during that period Was? 10 power-7 molar. If pH is 6 then what is the value of h What is the concentration? 10 power-6 molar and How did you derive this from this formula? H = anti - Do it. Now make the units of both the same. For this you can also write it as 1*10 power-6 You can use this value. and this is 10

26:35 Power is -6 only. take it to 1*10 power-6 You can. So you can see what the value is Was? 1 * 10 power-6 and final value How much is it? 1*10 Power-6 So Change How much did it cost? So the change in PA was 9*10 Power-6 this much change came in PA HN. As much The change in HN was equal to that of that acid The amount is in moles per liter. Salicylic What is the amount of acid? So we will say The amount of salicylic acid is 9 * 10 power -6 molar means moles per liter. in 100ml If asked, we will take it out in 100 ml. 10ml If I ask then we will take it out in 10 ml. So friends, if you have a little bit of information If you understand then think about this question. This will take some time but you can do it. I

27:23 I'm sure you can do it. what you need to do That any 15 ml of the said aqueous solution Is. Contains unknown amount of acetylcholine Is. We don't know how much acyl in 15 ml It's Collin. We don't know. again it is said How much was PA at that start? 7.65 How much was it? of pA 7.65 then an enzyme acetyl colon esterase he worked what did you do? breaks down the acetylcoline and whenever acyl choline breaks then H N Will meet. Change in PA due to HAI will come. PA changed to 6.87 How much did it cost after changing the PA? 6.87 Now you have to find out that from PA 7.65 PA is 6.87. then how much change in HN

28:11 Came? The molar change in HN is equal to The concentration was of acetyl choline. just that I want to find out this matter. It doesn't matter if the witnesses don't come There is nothing to worry about. son its my job Please tell me how it can be done. PA It is 7.65. If you take its antique then you will have it The initial HN concentration will be reached. It is 6.87. If you take its antithesis then you will have the final HN I got concentration. then you will see that finally The concentration of HN has increased. As much as HN The concentration has increased by that many moles of acetyl Colon was in 1 liter. what do we ask now Is? I have not asked for 1 litre. asked us how many were there in 15 ml? So in 1 litre If you know then you can extract it in 15 ml. If you know the quantity in 1 ml then you will divide it by 1000

28:58 So it will be known in 1 ml. He has to pay 15 to If you multiply it again you will get 15 ml Will go. Ok? I get scared as soon as I see the question yes. No, the logic is very simple. Look now How will you solve this? Look I've The solution is done so that you. So every price What does acyl colon give us? H ion Gives. Now how many moles do we have in 15 ml? We have to find out whether it is H ion or SL pellet. How much change came in PA? There was a change in PA Children 7.65 to 6.87 When PA was 7.65, we took its antilog. The amount of initial H ions we have is I. When the PA was 6.87, we had H The amount of ions came this much. Now both the amounts

29:43 which we have amount one and amount two Is. If you chose the amount of one out of two If you minus it then you will have HN I got concentration. The concentration of HN that came That is the concentration of acetyl choline. This There is a concentration of acetcholine in 1 liter. I found out about 1 litre here. We do not have any liters to find out. We have to find out in 15 ml. So To know whether 0 or 1 ml is in 15 ml Divide by 1000 to find out Take. then multiply it by 15 again or Direct to address in 15 ml 0.015 That means 15 ml means 0.015 litre. You can multiply this value by 0.015 Multiply it. As soon as the multiply If you do it you will get your answer. How much will it cost?

30:29 1.68 Which is somewhere in between. This method I will never ask you questions in the exam I don't tell you to do it. But even then if If your maths is strong then what is the concept I am telling you what it will feel like. The concept is very It was quite simple. This molecule changed the PA Did. Decreeded PA. What does it mean The solution was given by hn. how much in hn Did any change occur? From that, we found out how many There were molecules. so we only know had to do that how much change has happened in hn? 15ml In. So this will become our question. Ok? So what is the logic? very simple understanding I came, right? First you need to find out How many H ions were there in 7.65? then you We had to find out how many H ions were there in 6.87.

31:17 The H ions present here are called its HN ions. Subtract from it and you will get H How much change has occurred in the ion? As much as in HN What changed was acetylcholine. this which The amount is known to you, you can know it in 1 litre It has gone. If you want to find out in 15 ml then that Do the calculation accordingly. Ok? Value of anticlockwise there calculator It happens son. You can use them. Isn't it? Let us go If there is too much time during exam time Only then should one deal with such questions Needed Otherwise, it should not be put. Let's go now Friends, let's talk about another simple concept Let's talk to you. over there for antigue There is a calculator that can be pulled out Is. Friends, now we are talking about what is PK? I know about PK. PK's

32:03 The formula is pK is the negative log of KA. PK is the PA where acid gets 50% Dissociated. pK of the acid strength Tells about it. If less If it is PK then the acid is dissociating quickly Is. That's Stronger Acid. If the pK value is high So he is slowly dissociating. His Strength is low. i.e. PK and acid There is an inverse relationship in strength. Any What will be the pK value of the molecule It depends on whether he has How many acidic groups are there? like an amino Just take the acid, brother. then in amino acids At least one of these amino groups must be present

32:48 and if there is an amino acid then an acid group and It will definitely happen. plus many more of these amino acids There may be one or more groups in the side chain of which can be protonated and deprotonated. So Let me write all this down in acidic form. I would have written this in acidic form as well Am. Write it in COOH form also I will give it. let's write it in a COOH wali Form. Ok? So you have at least amino The acid must have two pK values. one alpha carboxylic group and one alpha Amino group. The friends you have alpha It is a carboxylic group. his values will see it ranges between 1.7 to 2.4 Is. So on average we can approximately calculate its value Let us consider this. If we have any

33:35 If numerical solution has to be done. and who alpha amino group is this alpha amino The pK values ​​of the groups ranged from 8.8 to 10.6. It ranges in. whose average value we Let's consider it around 9.5. When We have to do some numerical solution. So we understood the alpha of the children What will be the value of amino group? Us It was found out that the meaning of alpha acidic group Alpha Acidic Group Carboxylic Group What value of p should be taken? Back What are those amino acids left? whose R chain we need to know about Needed We should know this. Which ones? so acidic basic and a little Too much cysteine, serine, theodine, tyrosine

34:21 What should one know about? whose side chain has either or whose R chain what's in it? acidic group or those that have the side chain contains any amino group or Side chain has SH group or OH group Because it acts like acid inside them. Can. This can also donate H ions. this is also H can give ions. This can also donate H ions. This can also donate H ions. only in these You should know about amino acids. Ok? Let us go. Children Which amino acids will you remember? So Obviously those which are acidic, their PK The value will be the lowest and as you move towards the base will you keep moving towards or the one who is bigger than H N It is difficult to give their PK value

35:06 It will keep increasing. Friends, now the pK value of aseptic acid Talking about the side chain. how much would have been Is? 3.65 as many as there are days in a year 3.65 of aspartic acid Glutamic acid has 4.25 its side We are talking about the chain. now this is PK I am talking about three values. The imdazole ring of isin has The PK value is six. and this is important because this is the closest to the Physiological PA. that's why sdin is a The only amino acids which can act as a buffer ET from PA. Hello Bilal ji. Cysteine has a pK value of around 8.33.

35:55 More than that, tyrosine is around 10.07. then you also have this method one somebody OH group attached to the phenyl ring It happens. pK value of lysine and also 10.53 is too much Those whose pK value is higher can easily I will not say hi to you. I will keep it with me. I will not give it. Arginine 12.48 That's why we say Argin It is the strongest base and Sirin and Some are so fussy and so miserly So miserly that even if the PA becomes 13 then

36:42 we will also keep saying nana nana no no we will not If we don't give it, we won't give it to you HI will not provide their PK value syringes and it is more than 13 This brother of ours will not give me peace. Whatever happens, yes, beat the 13's with a lot of stick do it for around 14 only then their Deprotonation may occur. Ok? So What about aspartic acid? Single Letter codon d. Then what is it? of glutamic acid E. Then what is it? H. Then what is it? C. Then What is? Y. Then what is it? R. then what Is? Of. Then what is it? R. Then what is it? S Tea so this is the order

37:32 isn't it so what is it d e h c y k r s t See What is this Daily it is happening no i don't even know what to do next there is no need look at that what is this every day

38:20 Well friends, remember this many times One or two more things to remember in the series are that look in the meantime alpha carboxylic The group will come here first which will come first alpha carboxylic group first alpha carboxylic The value of the group will be around two And let's put one more in the middle here. Whose value would you put somewhere here? The value of alpha amino group is also added of alpha amino group which is approximately It would have been around 9.5. Just now if you If you remember these values ​​then you can do anything Not required. Now you have to find the value of P Whether you have given it or not, you have given it all The work will be done. Yes, definitely. the significance is that it will not act

39:05 As an acid. that's why we don't Considered as an acid. Let the significance be that it is not that why not considered Az acid. ok its sir sir its my recorded Your 6 month batch has ended yesterday. I talked to you officially once I want to contact you please sir I need Guidance is going to start live from today I will join the batch, yes, please do it Panda Then what is the problem, join the live batch from today From today onwards Pranshu Sir will guide you. I am ready for this and with Pranshu Sir Surprising And I will also come after 21 days Can I guide you in Biochemistry? Are you in trouble, Mr. Panda? You please come. Yours You are welcome. Having said

39:52 that we have to arrange the pK of the side chain According to. So how much does a sirin cost, brother? of Sirin We have it. We said that she had 13 Or even more than that. how much lysine Is? So brother, lysine is almost the same as yours Let's assume a value of around 10.5 for stdin How much is it then it is approximately around six How much is aspartate bro 3.65 Then the answer came out around We have Do you know what some great people said? I had gone there by heart, Lysin, I had even memorized it till here Some great people from amongst you have gone Some great and ascetic people

40:37 to Sirin It was considered more acidic than aspartate that the pK value of serine is higher than that of aspartate less because serine is a very strong acid Is. hey brother sirin if even with aspartate if there is more acid then it should be dissolved in acid You should have kept it. yes bilal Friends, now let's talk a little bit About the peptide bond. peptide bond it is form between an alpha carboxylic group and alpha Amino group. the alpha amino group is attacks the alpha carboxylic group On top of. And a new nine in between

41:23 A bond is formed. that is referred to as Peptide bond. in the peptide bond and always if you If you read the translation you will know a little more It will be clear friends in this way There is a P site. there's nobody there Contains peptide chains. And There is a site. There is no new amino acid It may have been so. Some new amino acid has arrived. Always The amino group of the new amino acid Attack this carboxylic group. Who will always be in reaction? Alpha Amino Who will the group attack? on the carboxylic group. Chemistry by the way It just works. Whether translation occurs at the N terminus from the C terminus, whether the C terminus is from the N be the terminus. attack the alpha amino group Who has to do it? So always keep the knife on the melon It will fall definitely. Melons will never grow in the air

42:10 that it falls on the knife. So who always reacts? will he do it? The alpha amino group will react With carboxylic group. Which one Which enzyme carries out this reaction? So this would cause a reaction during translation is peptidyl transferase. Papidal Transphase What is? If we talk about prokaryotes then 23s rRNA which is the large subunits of the ribosome Is a component of the unit. Isn't it? yes good Morning Esan. Partial double bond in these There is a character. neither the peptide bond There is neither a pure single bond nor a pure double bond There is a bond. Peptide bonds are generally in trans configuration It happens in. The bond angle is 180°. They It could also be sis. if it is proline then

42:56 any amino acid with children any amino acid If the proline bond is formed with acid is forming a peptide bond then which bond is it? how can it be? he could be sis too Is. And when cis happens then the value of omega How much will it be? What will be 0°? 0° When will Maharashtra set happen? A little video Search it. The time is coming as written, son. Don't do this. Concentrate on your studies now. The peptide bond is partially planar Because of the double bond character. the reason for which so almost six atoms they lie in a same Plane. About six atoms in one plane I do this. The peptide bond is polar Is. Therefore, peptide bonds are hydrogen bonded Also participates in bonding. Who

43:41 This is C double bed O group. it is always It will work as an acceptor. And there is NH group. Which will play the role of a hydrogen bond? Will work as a donor. Also provides UV absorption. focus on Keep this absorption in the far UV range does the show. So what are the important things? peptide bond is formed by peptidal transferase which is a riboenzyme in Present in Large Sabrit Were An Alpha Amino group which is present on a site it attacks the peptidyl chain which is on the P site peptide bond partial double Bond is a character. Peptide Bond Polar Is. Peptide Bonds Generally Trans Orientation takes place. This power is in proline

44:26 that cis peptide bond is also formed Can. Peptide bonds are activated by UV light Absorbs 190% in the far UV range And around 220. Peptide bond planar Is. Bring six atoms in the same plane Are. peptide bond hydrogen bonding Participates in. This is quite a few The important thing is the peptide bond. Yes it is possible to revise all units. Let us go Answer this question. Maharashtra set's Hopefully at 3:00 There will be a discussion, children. yes that's what I am going chand kumari Ask me all this later. Is it not possible son? I first time When I prepared for the exam, I got only 40

45:13 Around days i.e. around 45 days. But why do we even need to study 30 units? 8 9 I had prepared those units which is stronger for me then let's go 13 All my preparations are done. But when I too I was once a kid and was scared of JRF and I am talking about the first time I prepared Am. Yes Anavika will talk about it. Let us go. A Noble Organism Synthesizes Proteins using ribosome from C terminus to N Terminals which are easy to use Direction N terminus to Cirinus. in this case the main chain atom which act is a Nucleophile for the reaction. The nucleophile will remain what it is. They It will change a little. nucleophile which is The nucleus is loving and will remain the same. and who is he

45:58 we had the amino group which is the same He will stay brother, he will attack C Double B O H On the group. Whether it is from N10 to C10 or from C10 It doesn't matter if it's N10. although It doesn't make any difference, right? although It is he who has to attack. Who to attack Is? Brother whether it is N terminus or C terminus A nucleophilic attack will be carried out by the Can act as a nucleophile. Is No? very good. Well done. its answer is correct Answer option one. Let us go. Yes, I got JRF in my first attempt It also got cleared. rank in that though She did not come. But ah yes, for SPM The call had come. And that meant that I am among the toppers. the exact rank then that

46:45 Time doesn't come but since the rankings start coming If it happens then I always got a first right. well Come on now kids let's talk about some amino About acids. of the amino acids About the properties. I have checked my net more than 30 times and inside the ranking And the exam has been conducted every time till 2019. then If you have started giving the paper then now you have to give it There is no need for exam. Now it's just paper It becomes available. first paper available It did not happen. would have been available much later If it was there then I would have had to pay for it. well acidic amino acids Aspartic acid and latamic acid. Acidic amino acids are polar.

47:32 Negatively charged material is present on it Is. Friends, if acidic amino continuously if they are nearby then that strike Produce the entrance. putting a leg on hey what are you doing near me? Your charge is negative, mine is also negative Charge. Similarly basic peace is also based on lysine, an and It is studyin. this is also polar and positive There are charges. Basic ammonia also passed We can't even see each other when we are close by Do you make me hear that no, no, we are nobody It becomes possible. We don't get along. positive Positive charge does not form. Negative Negative charge cannot be created. Friends, The branch chain amino acids are valine, Leucine and isoleucine. these are also big scoundrels There are. This one also creates problems. Head Actually, if it were, it would be non-polar. away from the water they live. How are you?

48:18 Is hydrophobic. It is also essential. Non It is polar. But when do we make a mistake? When You are nearby. because their legs are crooked it occurs. There is a branch chain. then branch Because of the chain that strike intensity is more Let's produce it. These strict entrainments When do you produce it? When you are nearby. The basic ones should also be nearby. then branch If the chain amino acids were also close together, produce cylinders. why acidic Do you produce taxes? because same charge Same charges in these also. What is the reason for this? branch chains that cross each other of. Friends, there are three aromatic amino acids coming. phenyl alanine, tryptophan and Tyrosine. such as phenyl alanine and Tryptophan is also non-polar and essential is also there. You can make tyrosine from phenyl alanine and tyrosine is polar. Polar Generali

49:06 live on the surface. Non polar inside if you live but are adjacent to each other It also produces strict hindrance. Are. so nearby but also with side chains There should also be no amino acids. o how much Things to keep in mind brother in acidic pass Should not happen. not in basic passport Should be. containing branch chain amino acids Should not be nearby. Non Polar Amino Acids with Abnormal Side Chain Passages Should not be in. oh so many things The poor protein takes care of the It folds. oh brother oh brother friends Prone, I don't like that at all. Prone Non polar cyclic inside It is aliphatic cyclic. In which? Prolene Inside is non polar. and don't even ask that Don't even talk about that. He is the most Produces acidic entrance. To all

49:52 Our amino acids are useless. Who? Prone. He is around someone He says no. Glycine is not the only one which is largely responsible for the anger of the prone Can tolerate. all other amino acids Just fold my hands brother, let me remain prone. Now Let's talk about sulfur containing amino acids Acid. There are two sulfur containing amino acids. that is one is cysteine ​​and another is Mithyon. Cysteine ​​is our polar amino acid Is. You can say non essential, semi You can call it essential. methionin our It is non polar. poor cysteine ​​and methionine They are good kids. This is generally steric Does not produce hints. These stitens do not produce. Ok? so they do not produce the stick

50:38 Hints. And even poorer ones like this glycine It's done, Elenin is done. These are good kids. Thyonin became. He won't keep Thadin as a little rogue There is a child. asparaginate is there, glutamine is there Went. Isn't it? These are very low stick hints Let's produce it. there are very little chances that He will produce Strike Hintance. Yes cysteine ​​disulfide bonds also form He does. That is absolutely correct. Now answer this question. sir please Tell me. What time will the class be? 10 minutes Will have to skip class for this. yes, do it Take it son. No problem. I think 10:30 Till then I will try to finalize. Why? Seven There have been questions. There are total 20 questions

51:24 Combination of parts A and B. hey bro i don't have a phone number Brother Panda ji. There's only one way from me come to class to contact and because me and any other good counsellor better counsellor I am not. I can only teach children And I will teach you well. guarantee it I can. Let's talk further, kids yes deep praditya do it absolutely right proline can stay in alpha helix at n turn That is absolutely correct, it cannot happen on seats And I can't even stay in the middle at all It is correct, the class is not at 11:00 I had shifted Rani here at 11:00 The class is at 9:00 and tomorrow Remember, there is a class at 8:00 am from tomorrow Class is at 8:00am

52:21 You can take the contact number of IFS. IFS is 917268. Contact number of He will guide you in every way. Look, what is said in the question? A Cytoplasmic monomeric proteins containing a single non surface exposed Sistine was hidden inside Brother Sistine, who was hiding inside Cysteine ​​was hidden in a protein like this Suppose the protein is folding, which is inside It was hidden that there was someone called Sistine Bhaiya Ji Then what is being said, let's read further Brother, let's see what is being said once For however upon mutation of cysteine ​​to Isoleucine isoleucine in place of cysteine I brought isoleucine when I came How is it branched

53:10 Now replace cysteine ​​with branch It gets precipitated. o ho precipitate It happens. but in place of cysteine If you bring alanine then it remains soluble. These it is getting soluble and it remains precipitated Used to be. Isn't it a brush, it's precipitating Is. Sistine to Allen Leads to a Soluble End Functional. Doesn't matter. So what Is the anser correct? So precipitate is forming. One thing we also We can say that in comparison with Elenin Isolation is also more non-polar. Hydrophobicity will also increase. Cysteine There was a polar. It is very much non-polar. cysteine ​​no your asterisk hints I did not produce it. O in solution Brother, this is acidic hint with branch chain He will also produce. So what's going on? Change is happening. A couple of changes are taking place. Firstly non polar is coming from polar, brother.

53:58 and what other change is coming that unbranch The branch is coming from. Two changes are taking place. The branch is coming from the unbranch. So these Your answer should be around . your answer It should be around these. Ok? Tahseen son. Let us go. son muted isolate alternate charge no There is no issue regarding the charges. 16 Mutated Alanine Alternate Charge No Charge There is no problem at all. 16 Mute alanine would be a cosmogony if Because of Elenin Then there is a difference. Why is it not falling? Cysteine ​​Mutated to Isolation Causes Steric Hints in the Core of Proteins

54:44 While mutation to alanine does not occur. Oho Brother, this will be the answer. This question itself is screaming I will answer that I will answer that I will answer that. Let's talk further. Friends, now what are more tips? what are The secondary structures? about that too You should know that when there is more than one Secondary structures are intact with each other If we do that then we call that higher level structure What are super secondary structures or motives? Can be done. As you can see there are more than one beta sheet this is one beta sheet this is another beta sheet third beta sheet fourth beta sheets together I am getting intermediate. So many of your motives Let's know about it. As

55:29 You have a helix turn helix motive Can. A hair pin could be the motive. Friends, this structure that has been built, that Is a Greek key motive. This includes four anti- parallel beta sheet, that is the Greek key Motive. inside everyone here you have the From one side, suppose the N terminus of the N turn is It is going on like this. then walk like this walk like this Sea Turns came back. and this is its mirror The image is. Look at this also on the outside Who is it? C Terms. And which one is on this side? N. Turn. In this also you will see the car from here It has started, right? You are from here, right? So Their orientation is almost the same An attempt was made to tell you that this is with you the first one is done this is the second beta sheet Neither is it the first nor did it become the first like this This is the second one, this is the third one, this is the fourth one

56:15 It has been told here also that this is the first this became the second one this became the third one and This is the fourth one, these two are almost the same The structure is a Greek motif friends from one sequences with higher polytype changes in There is also a variation in it that too in a similar way You can form the motive. The motives are related to the secondary structure So there are more but from the tertiary structure It is less. Because the tertiary structure is overall Folding of proteins. What is? Overall Folding of Proteins. If you are asked what is the total length of the motive what will it be? So what do we need to know? Connect the motives with each other. from that to your The pass length will be known. like which of these If you had this motive parallel to yours

57:00 Near If there were such parallel sheets, then the length of that motive It would have been more. Why is the length of the motive longer? Would it be? Because then how do you connect? Would it have happened? connect in the middle like this We have to connect here and there Would have fallen. It had to be connected from here to there. here It would have connected here. what should be its length Caste? It would have become too much. If friends If there is an antiparallel beta sheet then its motive is which will form a polypeptide because anti What is in antiparallel inside a peptide What happens is that it turns straight. straight like this it turns around and becomes like this then its The length will be the shortest. So keep in mind that More and more anti inside motive Parallel beta sheet comes that motive The length will be the shortest because anti-parallel I have to take a straight turn. Parallel Beta what do you have to do in the sheet brother that this beta If you have a sheet and want to make it parallel then make it parallel That it will have to be rotated like this. then again you parallel

57:45 You can make it. You will always have its length It will be more. This happened en turnus. This is C Turnus is done. If we try to obtain parallel beta sheets, then you have to find the polypeptide in the middle Having a large flexible region It will have to be done. so you will see biological In most places in the system your children Only anti-parallel beta sheets are found near Is. because anti parallel beta sheets Hydrogen is more stable because of the bonding and a beta inside it to connect one beta sheet to another Only four amino acids are sufficient for connect them to what is called beta turn Can be done. How do you know the sequence, son? Sequence This is discovered through protein sequencing. Is No? By doing sequencing, we find out which have different motives they perform a Different functions. Now let's see how many questions you have.

58:32 The simple question is whether you have motives I have given it to you and you have to tell me according to the length Have to arrange it from. Ok? I don't have a recorded class Is. Should I watch that or the YouTube live? My I don't have any recorded classes. He Should I watch it when YouTube is not live? How will you see that, son? And if it is, then you Check them out and check out YouTube too Son. Let us go So what do you want to see brother? This total Together, the story is hidden somewhere here It has happened. The story is somewhere in this. see how do you connect it, it's always there

59:17 to connect that to the N terminal Let's change the color of one loop a little like this So that you will enjoy seeing this I connected this here and this too I connected it directly from here, so let's do this Peptide one is the shortest, so the smallest will be If you want to connect inside this then If you have to connect it back to the tail later bring it here to connect on the tail You will then have to connect this to the tail as well It will have to be brought here. That means this is the most It became the smallest. This became the largest. because one's head is bigger than the other's tail will clash. Its head will collide with its tail. its head back its tail back its head back

1:00:03 Will have to clash with the tail. This is quite long Went. Look at this brother. its head its tail Make him fight with him. make its head like this It will have to be done. so there's a small loop in it four the amino acid and the rest a slightly longer loop Is. What is inside this too brother? a small There is a loop and there is a bigger loop on it. aye this big There is a loop. So the situation of three and four is the same Is. Situation of three and four is same. Three And Four's situation is the same. So give it two There are big loops. There is a U term in it, There is a loop. There are two quick U-turns in this Those. Ok? come on so what is the answer that most What happened to the younger brother? Peptide

1:00:49 Forest. Peptide three became bigger than that Peptide Four. and who has the longest length Went? Peptide Two Key. So what should we arrange Had to do it? Regarding the length. of length They had to arrange it accordingly. why antima why your question I am unable to answer? It may have been The reason for this could be the fact that I have mentioned above He must have gone away so I am unable to give it to you Son, what is your relation with me and what am I relation with you? You are my child, will you clear JRF? what problem could I have with you be a good boy to me I did the exam well, it was not like this, very good Well done B.Sc. studies are not of NET level and there is no problem in hindi medium

1:01:35 Son, I was also from Hindi medium, so no There is nothing to be ashamed of and scared of It doesn't matter even if you read it now Isn't it? Start building a base with YouTube and once the base is ready Then any IFS class recorded or Join the live class. Your M.Sc. There will be profit in it and profit in the net as well will be. That is a suggestion from my side. Let us go Isn't it? Don't go straight. A little bit on YouTube First get acclimatized for IFS in a manner. and if you get acclimatized then Then come north inside the race. now kids we are talking About CD Spectroscopy. Correct Is? Yes, I will give you the ideals too. CD Far UV range in spectroscopy is used.

1:02:22 Isn't it? From the far UV range. Far UV Range Who absorbs it? So Far UV Peptide Absorbs Range To Bonds. when different types of secondary There are structures the orientation of the peptide bonds in them It varies. So we're in the far UV range From 190 or from 180 let's say 250 Nm By giving different circularly polarized light Let's see. and we check that the different What is electricity per wavelength? V Check The Electricity at Different Wavelengths. Check electricity on different wavelengths He does. When you look carefully you will It turns out that the movement is of 190 Nm comes around and the other commotion that comes Comes around 220 Nm. these are the two Major Wavelengths of a Circular Polarized

1:03:10 light. which is basically absorbed by the peptide bonds. where you have a very big There is a commotion to be seen. Ok? Let us go. Friends n2 which are transitions near 220 nm The reason for this is n to pi star transitions and the transitions on the lower side from 210 nm Hote Hain That Is Due To Pi To Pi Star These are transitions, which are energy levels This is happening because of transitions. Friends A simple way to identify alpha helix There is a way. Inside Alpha Elix you will first find One positive peak appears and two sharp Negative peaks appear. Isn't it? To you The first one is a positive peak approx.

1:03:56 and two negative peaks one around 190 nm one at 208 nm and one at 222 nm It is available. He must understand that this what is it brother? This structure is alpha Alex. What is the structure like? Alphafix. Kids, this is not just PYQ. This session is going to be of great use. You Isn't it? Specially if you want my paid batch Or whether you are a member of YouTube, everyone Very useful session for this type of curve if he comes then he should understand whose Hello Sonam ji from Alfalis Friends So how to identify alphas a pick one on 190 one on 208 and one on 222 from that you know

1:04:42 will work Friends, in this way the peak of beta sheet the way to identify it is that an approximately peaks around 192 and a low around 218 a negative peak around 100 ohms before a positive one A peak appears and then a negative peak It is visible. so while in alpha what Was? There was one positive peak and two negative peaks Were. one can assume around 208 and one Around 222. Here 216 to 218 Nm A peak is visible nearby. Although The positive peak is also of slightly lesser height. The negative peak is also of low height beta sheet In. Friends who is a random coil when the secondary of some method If the structure is not formed then it is peeled off

1:05:29 If you look at it, it will look exactly like the reverse of a beta sheet It might have been done. almost you will find it as a Reverse. This will give you a positive peak first and then a negative peak will appear will give. it is would be almost a reversal of a Beta sheet which shows you the first negative peak will give inside electricity that too almost around 192 to 195 nm and which is slightly A poor person will appear with a positive peak You will also see a positive peak, children Around 218 nm approx. Friends, if there is any structure among these We are secondary to some unknown person You can find out about the structure. That is, if we see some such structure then we can say I will give it to you brother, this is Alphabets. something like that

1:06:14 If the structure is visible then something like this The structure looks sharp, something like this

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